Products list
        Online Service
        Your present location:Home Pictures >> TOSOH Column
        Products

        TSKgel PW Columns for Analysis of Water-Soluble Polymers

        TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.

        The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.

        The figure to the left shows the calibration curves for all TSKgel PW-type columns. Suitable solvents for various polymer samples are shown in the table below.

         

        说明: https://www.separations.asia.tosohbioscience.com/Image%20Library/TBJS/img/Columns/SEC/SEC_PW_Curves.jpg?code=a1af9c5f-8268-46ed-8711-79c0aba0b46c

        Specialty TSKgel PW-type Columns

        The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples. TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides. TSKgel G1000PW is the preferred column for analysis of low MW oligomers.

        Type of Polymer 

        Typical Sample

        Suitable Eluent

        Nonionic hydrophilic

        polyyethylene glycol

        soluble starch, methyl cellulose, 

        pullulan dextran, hydroxyethyl cellulose 

        polyvinyl alcohol, polyacrylamide

        distilled water
        0.01 mol/L NaOH
        20% DMSO
        Buffer or salt solution (e.g. 0.1-0.5 mol/L NaNO3)

        Nonionic Hydrophobic

           
             

        polyvinylpyrrolidone

        Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)

        Anionic Hydrophilic

        sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate

        Buffer or salt solution (e.g. 0.1 mol/L NaNO3)

        Anionic Hydrophobic

        sulfonated lignin sodium salt, 
        sodium polystyrenesulfonate
         

        Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3)

        Cationic Hydrophilic

        glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt

        0.5M acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3

        Cationic Hydrophobic

        poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt

        0.5 mol/L acetic acid with 0.3 mol/L Na2SO4

        Amphoteric Hydrophilic

        peptides, proteins, poly- and oligosaccharides, DNA, RNA

        Buffer or salt solution (e.g. 0.1 mol/L NaNO3)

        Amphoteric hydrophobic
         

        blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides

        Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA)

         

        TSKgel G2000PW

        Base Material:

        hydroxylated polymethacylate

        Particle Size (mean):

        12 µm

        Pore size (mean):

        12.5 nm

        Calibration Range (polyethylene glycols and oxides):  

        up to 2,000 Da

        Max. % Organic solvent:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particle size

        Housing material

        ID (mm)

        Length (cm)

        0005761

        TSKgel G2000PW

        12 µm

        Stainless Steel

        7.5

        30

        0005105

        TSKgel G2000PW

        12 µm

        Stainless Steel

        7.5

        60

        0006763

        Guard Column for P/N 0005761 and 0005105

        13 µm

        Stainless Steel

        7.5

        7.5

         

        TSKgel G2500PW

        Base Material:

        hydroxylated polymethacrylate

        Particle Size (mean):

        12 µm and 17 µm

        Pore size (mean):

        <20 nm

        Calibration Range (polyethylene glycols and oxides):  

        up to 3,000 Da

        Max. % Organic:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particle size

        Housing material

        ID (mm)

        Length (cm)

        0008028

        TSKgel G2500PW

        12 µm

        Stainless Steel

        7.5

        30

        0008029

        TSKgel G2500PW

        12 µm

        Stainless Steel

        7.5

        60

        0006762

        Guard Column for P/N 0008028, 0008029

        13 µm

        Stainless Steel

        7.5

        7.5

        0016248

        TSKgel G2500PW

        17 µm

        Stainless Steel

        21.5

        30

        0008030

        TSKgel G2500PW

        17 µm

        Stainless Steel

        21.5

        60

        0006758

        Guard Column for P/N 0016248, 0008030

        17 µm

        Stainless Steel

        21.5

        7.5

         

        TSKgel G3000PW

        Base Material:

        hydroxylated polymethacylate

        Particle Size (mean):

        12 µm and 17 µm

        Pore size (mean):

        20 nm

        Calibration Range (polyethylene glycols and oxides):  

        up to 50,000 Da

        Max. % Organic:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particle size

        Housing material

        ID (mm)

        Length (cm)

        0005762

        TSKgel G3000PW

        12 µm

        Stainless Steel

        7.5

        30

        0005106

        TSKgel G3000PW

        12 µm

        Stainless Steel

        7.5

        60

        0006762

        Guard Column for P/N 0005762, 0005106

        13 µm

        Stainless Steel

        7.5

        7.5

        0016249

        TSKgel G3000PW

        17 µm

        Stainless Steel

        21.5

        30

        0005151

        TSKgel G3000PW

         17 µm

         Stainless Steel

        21.5

        60 

        0006758

        Guard Column for P/N 0016249, 0005151

        17 µm

        Stainless Steel

        21.5

        7.5

         

        TSKgel G4000PW

        Base Material:

        hydroxylated polymethacylate

        Particle Size (mean):

        17 µm

        Pore size (mean):

        50 nm

        Calibration Range (polyethylene glycols and oxides):  

        up to 300,000 Da

        Max. % Organic:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particle size

        Housing material

        ID (mm)

        Length (cm)

        0005763

        TSKgel G4000PW

        17 µm

        Stainless Steel

        7.5

        30

        0005107

        TSKgel G4000PW

        17 µm

        Stainless Steel

        7.5

        60

        0006762

        Guard Column for P/N 0005763, 0005107

        13 µm

        Stainless Steel

        7.5

        7.5

        0016250

        TSKgel G4000PW

        20 µm

        Stainless Steel

        21.5

        30

        0005152

        TSKgel G4000PW

        20 µm

         Stainless Steel

        21.5

         60

        0006758

        Guard Column for P/N 0016250, 0005152

        17 µm

        Stainless Steel

        21.5

        7.5

         

        TSKgel G5000PW

        Base Material:

        hydroxylated polymethacylate

        Particle Size (mean):

        17 µm

        Pore size (mean):

        100 nm

        Calibration Range (polyethylene glycols and oxides):  

        up to 1,000,000 Da

        Max. % Organic:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particle size

        Housing material

        ID (mm)

        Length (cm)

        0005764

        TSKgel G5000PW

        17 µm

        Stainless Steel

        7.5

        30

        0005108

        TSKgel G5000PW

        17 µm

        Stainless Steel

        7.5

        60

        0006762

        Guard Column for P/N 0005764, 0005108

        13 µm

        Stainless Steel

        7.5

        7.5

        0016251

        TSKgel G5000PW

        20 µm

        Stainless Steel

        21.5

        30

        0005153

        TSKgel G5000PW

        20 µm

        Stainless Steel

        21.5

        60

        0006758

        Guard Column for P/N 0016251, 0005153

        17 µm

        Stainless Steel

        21.5

        7.5

         

        TSKgel G6000PW

        Base Material:

        hydroxylated polymethacylate

        Particle Size (mean):

        17 µm

        Pore size (mean):

        >100 nm

        Calibration Range (polyethylene glycols and oxides):  

        up to 8,000,000 Da

        Max. % Organic:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particle size

        Housing material

        ID (mm)

        Length (cm)

        0005765

        TSKgel G6000PW

        17 µm

        Stainless Steel

        7.5

        30

        0005109

        TSKgel G6000PW

        17 µm

        Stainless Steel

        7.5

        60

        0006762

        Guard Column for P/N 0005765 and 0005109

        13 µm

        Stainless Steel

        7.5

        7.5

        0020024

        TSKgel BioAssist G6PW

        17 µm

        PEEK

        7.8

        30

         

        TSKgel GMPW

        Base Material:

        hydroxylated polymethacylate

        Particle Size (mean):

        17 µm

        Pore size (mean):

        <10 - 100 nm

        Calibration Range (polyethylene glycols and oxides):  

        500 - 8,000,000 Da

        Max. % Organic:

        20%

        Shipping Solvent:

        water

         

        P/N

        Description

        Particlesize

        Housingmaterial

        ID(mm)

        Length(cm)

        0008026

        TSKgel GMPW

        17 µm

        Stainless Steel

        7.5

        30

        0008027

        TSKgel GMPW

        17 µm

        Stainless Steel

        7.5

        60

        0006762

        Guard Column for P/N 0008026 and 0008027

        13 µm

        Stainless Steel

        7.5

        7.5