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TSKgel SWXL Columns for Protein Analysis
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TSKgel SWXL Columns for Protein Analysis
TSKgel SWXL columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
TSKgel SWXL-type columns contain smaller particles than TSKgel SW-type columns; 5 and 8 μm versus 10 and 13 μm. As the TSKgel SW-type columns, TSKgel SWXL columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWXL-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.
Calibration Curves The Figure shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SWXL-type columns. The tables below include the molecular weight ranges for TSKgel G2000SWXL, G3000SWXL and G4000SWXL columns for globular proteins, polyethylene oxides and dextrans that can, at leastpartially, access the pores. |
TSKgel G2000SWXL
Base Material: |
Silica |
Particle Size(mean): |
5 µm |
Pore Size(mean): |
12.5 nm |
Functional Group: |
Diol |
pH Stability: |
2.5 - 7.5 |
Calibration Range(globular proteins): |
5,000-150,000 |
Calibration Range(dextrans): |
1,000 - 3 x 104 |
Calibration Range(polyethylene glycols and oxides): |
500 - 15,000 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0020027 |
TSKgel BioAssist G2SWXL |
5μm |
PEEK |
7.8 |
30 |
0018008 |
Guard Column for P/N 0020027 |
7μm |
PEEK |
6 |
4 |
0008540 |
TSKgel G2000SWXL |
5μm |
Stainless Steel |
7.8 |
30 |
0008543 |
Guard Column for P/N 0008540 |
7μm |
Stainless Steel |
6 |
4 |
0008544 |
TSKgel SWXL Top-Off for SWXL, 1g |
5μm |
TSKgel G3000SWXL
Base Material: |
Silica |
Particle Size(mean): |
5 μm |
Pore Size(mean): |
25 nm |
Functional Group |
Diol |
pH Stability |
2.5 - 7.5 |
Calibration Range(globular proteins): |
1 x 104 - 5 x 105 |
Calibration Range(dextrans): |
2,000 - 7 x 104 |
Calibration Range(polyethylene glycols and oxides): |
1,000 - 3.5 x 104 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0020026 |
TSKgel BioAssist G3SWXL |
5μm |
PEEK |
7.8 |
30 |
0018008 |
Guard Column for P/N 0020026 |
7μm |
PEEK |
6 |
4 |
0008541 |
TSKgel G3000SWXL |
5μm |
Stainless Steel |
7.8 |
30 |
0008543 |
Guard Column for P/N 0008541 |
7μm |
Stainless Steel |
6 |
4 |
0008544 |
TSKgel SWXL Top-Off for SWXL, 1g |
5μm |
TSKgel G4000SWXL
Base Material: |
Silica |
Particle Size(mean): |
8 µm |
Pore Size(mean): |
45 nm |
Functional Group: |
Diol |
pH Stability: |
2.5 - 7.5 |
Calibration Range(globular proteins): |
20,000 - 7 x 106 |
Calibration Range(dextrans): |
4,000 - 5 x 105 |
Calibration Range(polyethylene glycols and oxides): |
2,000 - 2.5 x 105 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0020025 |
TSKgel BioAssist G4SWXL |
8μm |
PEEK |
7.8 |
30 |
0018008 |
Guard Column for P/N 0020025 |
PEEK |
6 |
4 |
|
0008542 |
TSKgel G4000SWXL |
8μm |
Stainless Steel |
7.8 |
30 |
0008543 |
Guard Column for P/N 0008542 |
7μm |
Stainless Steel |
6 |
4 |
0008544 |
TSKgel Top-Off for SWXL, 1g |
5μm |
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