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TSKgel SW Columns for Protein Analysis

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
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TSKgel SW Columns for Protein Analysis

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

The calibration curves are shown for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The table summarizes the molecular weight ranges for TSKgel G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWXL and SuperSW-type columns.

Greenherbs

TSKgel G2000SW

Base Material:

Silica

Particle Size(mean):

10 µm and 13 µm

Pore size(mean):

12.5 nm

Functional Group:

Diol

pH Stability:

2.5 - 7.5

Calibration Range(globular proteins):

5,000 - 100,000 Da

Calibration Range(dextrans):

1,000 - 3 × 104

Calibration Range(polyethylene glycols and oxides):

500 - 15,000

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0005788

TSKgel G2000SW

10μm

Stainless Steel

7.5

30

0005102

TSKgel G2000SW

10μm

Stainless Steel

7.5

60

0005371

Guard column for P/N 0005788 and 0005102

Stainless Steel

7.5

7.5

0006727

TSKgel G2000SW

13μm

Stainless Steel

21.5

30

0005146

TSKgel G2000SW

13μm

Stainless Steel

21.5

60

0005758

Guard Column for P/N 0006727 and 0005146

Stainless Steel

21.5

7.5

0006819

TSKgel SW Top-Off for 7.5mm ID SW, 1g

10μm

TSKgel G3000SW

Base Material:

Silica

Particle Size(mean):

10 µm and 13 µm

Pore Size(mean):

25 nm

Functional Group:

Diol

pH Stability:

2.5 - 7.5

Calibration Range(globular proteins):

10,000 - 500,000

Calibration Range(dextrans):

2,000 - 7 x 104

Calibration Range(polyethylene glycols and oxides)

1,000 - 3.5 x 104

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0008800

TSKgel G3000SW

10μm

Glass

8

30

0008805

Guard Column for P/N 0008800

Glass

8

4

0005789

TSKgel G3000SW

10μm

Stainless Steel

7.5

30

0005103

TSKgel G3000SW

10μm

Stainless Steel

7.5

60

0005371

Guard Column for P/Ns 0005789, 0005103

Stainless Steel

7.5

7.5

0006819

TSKgel Top-Off for 7.5mmID SW, 1 g

10μm

Stainless Steel

0006728

TSKgel G3000SW

13μm

Stainless Steel

21.5

30

0005147

TSKgel G3000SW

13μm

Stainless Steel

21.5

60

0005758

Guard Column for P/Ns 0006728, 0005147

Stainless Steel

21.5

7.5

TSKgel G40000SW

Base Material:

Silica

Particle Size(mean):

13 µm and 17 µm

Pore Size(mean):

45 nm

Functional Group:

Diol

pH Stability:

2.5 - 7.5

Calibration Range(globular proteins):

20,000 - 7 × 106

Calibration Range(dextrans):

4,000 - 5 x 105

Calibration Range(polyethylene glycols and oxides):

2,000 - 2.5 x 105

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0008801

TSKgel G4000SW

13μm

Glass

8

30

0008805

Guard column for P/N 0008801

Glass

8

4

0005790

TSKgel G4000SW

13μm

Stainless Steel

7.5

30

0005104

TSKgel G4000SW

13μm

Stainless Steel

7.5

60

0005371

Guard column for P/N 0005790 and 0005104

Stainless Steel

7.5

7.5

0006819

TSKgel SW Top-Off, 1g for P/N 005790, 0005104

10μm

0006729

TSKgel G4000SW

17μm

Stainless Steel

21.5

30

0005148

TSKgel G4000SW

17μm

Stainless Steel

21.5

60

0005758

Guard column for P/N 0006729, 0005148

Stainless Steel

21.5

7.5

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