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TSKgel SW Columns for Protein Analysis
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TSKgel SW Columns for Protein Analysis
TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.
The calibration curves are shown for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The table summarizes the molecular weight ranges for TSKgel G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWXL and SuperSW-type columns.
TSKgel G2000SW
Base Material: |
Silica |
|
Particle Size(mean): |
10 µm and 13 µm |
|
Pore size(mean): |
12.5 nm |
|
Functional Group: |
Diol |
|
pH Stability: |
2.5 - 7.5 |
|
Calibration Range(globular proteins): |
5,000 - 100,000 Da |
|
Calibration Range(dextrans): |
1,000 - 3 × 104 |
|
Calibration Range(polyethylene glycols and oxides): |
500 - 15,000 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0005788 |
TSKgel G2000SW |
10μm |
Stainless Steel |
7.5 |
30 |
0005102 |
TSKgel G2000SW |
10μm |
Stainless Steel |
7.5 |
60 |
0005371 |
Guard column for P/N 0005788 and 0005102 |
Stainless Steel |
7.5 |
7.5 |
|
0006727 |
TSKgel G2000SW |
13μm |
Stainless Steel |
21.5 |
30 |
0005146 |
TSKgel G2000SW |
13μm |
Stainless Steel |
21.5 |
60 |
0005758 |
Guard Column for P/N 0006727 and 0005146 |
Stainless Steel |
21.5 |
7.5 |
|
0006819 |
TSKgel SW Top-Off for 7.5mm ID SW, 1g |
10μm |
TSKgel G3000SW
Base Material: |
Silica |
Particle Size(mean): |
10 µm and 13 µm |
Pore Size(mean): |
25 nm |
Functional Group: |
Diol |
pH Stability: |
2.5 - 7.5 |
Calibration Range(globular proteins): |
10,000 - 500,000 |
Calibration Range(dextrans): |
2,000 - 7 x 104 |
Calibration Range(polyethylene glycols and oxides) |
1,000 - 3.5 x 104 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0008800 |
TSKgel G3000SW |
10μm |
Glass |
8 |
30 |
0008805 |
Guard Column for P/N 0008800 |
Glass |
8 |
4 |
|
0005789 |
TSKgel G3000SW |
10μm |
Stainless Steel |
7.5 |
30 |
0005103 |
TSKgel G3000SW |
10μm |
Stainless Steel |
7.5 |
60 |
0005371 |
Guard Column for P/Ns 0005789, 0005103 |
Stainless Steel |
7.5 |
7.5 |
|
0006819 |
TSKgel Top-Off for 7.5mmID SW, 1 g |
10μm |
Stainless Steel |
||
0006728 |
TSKgel G3000SW |
13μm |
Stainless Steel |
21.5 |
30 |
0005147 |
TSKgel G3000SW |
13μm |
Stainless Steel |
21.5 |
60 |
0005758 |
Guard Column for P/Ns 0006728, 0005147 |
Stainless Steel |
21.5 |
7.5 |
TSKgel G40000SW
Base Material: |
Silica |
Particle Size(mean): |
13 µm and 17 µm |
Pore Size(mean): |
45 nm |
Functional Group: |
Diol |
pH Stability: |
2.5 - 7.5 |
Calibration Range(globular proteins): |
20,000 - 7 × 106 |
Calibration Range(dextrans): |
4,000 - 5 x 105 |
Calibration Range(polyethylene glycols and oxides): |
2,000 - 2.5 x 105 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0008801 |
TSKgel G4000SW |
13μm |
Glass |
8 |
30 |
0008805 |
Guard column for P/N 0008801 |
Glass |
8 |
4 |
|
0005790 |
TSKgel G4000SW |
13μm |
Stainless Steel |
7.5 |
30 |
0005104 |
TSKgel G4000SW |
13μm |
Stainless Steel |
7.5 |
60 |
0005371 |
Guard column for P/N 0005790 and 0005104 |
Stainless Steel |
7.5 |
7.5 |
|
0006819 |
TSKgel SW Top-Off, 1g for P/N 005790, 0005104 |
10μm |
|||
0006729 |
TSKgel G4000SW |
17μm |
Stainless Steel |
21.5 |
30 |
0005148 |
TSKgel G4000SW |
17μm |
Stainless Steel |
21.5 |
60 |
0005758 |
Guard column for P/N 0006729, 0005148 |
Stainless Steel |
21.5 |
7.5 |
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