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TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis

TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
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TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis

TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristic

TSKgel SuperSW-type columns contain smaller particles than TSKgel SWXL-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements

It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. See Separation Report 95 for recommendations.

Calibration Curves

The figure to the right shows the calibration curves for globular proteins for the two TSKgel SuperSW-type columns in comparison with those for the corresponding TSKgel SWXL columns.

Greenherbs

TSKgel SuperSW2000

Base Material:

Silica

Particle Size(mean):

4 µm

Pore Size(mean):

12.5 nm

Functional Group:

Diol

pH Stability:

2.5 - 7.5

Calibration Range(globular proteins):

5,000 - 150,000

Calibration Range(dextrans):

1,000 - 3 x 104

Calibration Range(polyethylene glycols and oxides):

500 - 15,000

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0018674

TSKgel SuperSW2000

4µm

Stainless Steel

4.6

30

0018762

Guard Column for P/N 0018674

Stainless Steel

4.6

3.5

TSKgel SuperSW3000

Base Material:

Silica

Particle Size(mean):

4 µm

Pore Size(mean):

25 nm

Functional Group:

Diol

pH Stability:

2.5 - 7.5

Calibration Range(globular proteins):

1 x 104 - 5 x 105

Calibration Range(dextrans):

2,000 - 7 x 104

Calibration Range(polyethylene glycols and oxides):

1,000 - 3.5 x 104

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0018675

TSKgel SuperSW3000

4µm

Stainless Steel

4.6

30

0018762

Guard Column for P/N 0018675

Stainless Steel

4.6

3.5

0021485

TSKgel SuperSW3000

4µm

Stainless Steel

2

30

0021845

TSKgel SuperSW3000

4µm

Stainless Steel

1

30

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