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TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis
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TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis
TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristic
TSKgel SuperSW-type columns contain smaller particles than TSKgel SWXL-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.
HPLC System Requirements
It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. See Separation Report 95 for recommendations.
Calibration Curves
The figure to the right shows the calibration curves for globular proteins for the two TSKgel SuperSW-type columns in comparison with those for the corresponding TSKgel SWXL columns.
TSKgel SuperSW2000
Base Material: |
Silica |
Particle Size(mean): |
4 µm |
Pore Size(mean): |
12.5 nm |
Functional Group: |
Diol |
pH Stability: |
2.5 - 7.5 |
Calibration Range(globular proteins): |
5,000 - 150,000 |
Calibration Range(dextrans): |
1,000 - 3 x 104 |
Calibration Range(polyethylene glycols and oxides): |
500 - 15,000 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0018674 |
TSKgel SuperSW2000 |
4µm |
Stainless Steel |
4.6 |
30 |
0018762 |
Guard Column for P/N 0018674 |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperSW3000
Base Material: |
Silica |
Particle Size(mean): |
4 µm |
Pore Size(mean): |
25 nm |
Functional Group: |
Diol |
pH Stability: |
2.5 - 7.5 |
Calibration Range(globular proteins): |
1 x 104 - 5 x 105 |
Calibration Range(dextrans): |
2,000 - 7 x 104 |
Calibration Range(polyethylene glycols and oxides): |
1,000 - 3.5 x 104 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0018675 |
TSKgel SuperSW3000 |
4µm |
Stainless Steel |
4.6 |
30 |
0018762 |
Guard Column for P/N 0018675 |
Stainless Steel |
4.6 |
3.5 |
|
0021485 |
TSKgel SuperSW3000 |
4µm |
Stainless Steel |
2 |
30 |
0021845 |
TSKgel SuperSW3000 |
4µm |
Stainless Steel |
1 |
30 |
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