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TSKgel SuperHZ Columns for Reduced Polymer Adsorption and Limited Solvent Range
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TSKgel SuperHZ Columns for Reduced Polymer Adsorption and Limited Solvent Range
The TSKgel SuperHZ column line consists of five columns with different pore sizes, TSKgel SuperHZ1000 through TSKgel SuperHZ4000, and three columns with an extended linear range of the calibration curve, TSKgel SuperHZM-N, SuperHZM-M and SuperHZM-H. The calibration curves are shown below.
Main characteristics:
TSKgel SuperHZ columns have been developed for High Throughput, High Efficiency GPC applications such as those encountered in combinatorial chemistry experiments.
The benefits of TSKgel SuperHZ columns over conventional TSKgel HXL columns are shown in the following chromatogram.
Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
Maximum operating temperature for TSKgel SuperHZ-type columns is 60 ºC for TSKgel SuperHZ1000, SuperHZ2000, SuperHZ2500 and SuperHZ3000, and 80 ºC for the remaining columns in the product line. See TSKgel SuperH-type columns for operating temperatures of 140 ºC.
Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents as shown below.
Calibration Curves for TSKgel SuperHZ Columns
Solvent compatibility for TSKgel SuperHZ columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Recommended Solvent |
Application |
THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate, |
N,N-Dimethylformamide(DMF) +/- 5mM LiBr |
polyvinyl chloride, polyvinyl fluoride, urea resins, |
o-Dichlorobenzene(ODCB) |
polyethylene, polypropylene |
Chloroform |
polycarboxylic ether, acrylic resin, |
m-Cresol/Chloroform |
nylon, polyester, polyamide, |
Toluene |
polybutadiene, polysiloxane |
Beware of extra-column bandbroadening
Column dimensions are 6 mm ID x 15 cm and 4.6 mm ID x 15 cm versus 7.8 mm ID x 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4(6 mm ID) and a factor of 5.8(4.6 mm ID) versus the same component eluting from a corresponding TSKgel HXL column. Thus, your HPLC system may require optimization of components that can give rise to extra-column bandbroadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.
Columns with linear calibration curves
The TSKgel SuperHZ product line contains three linear, or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed-bed columns feature increasing linear calibration ranges, from TSKgel SuperHZM-L, SuperHZM-N, SuperHZM-H.
Shipping Solvent |
Can Be Replaced With |
Tetrahydrofuran |
benzene, chloroform, toluene, xylene, |
Acetone** |
carbon tetrachloride4, o-dichlorobenzene, dimethylformamide, |
Chloroform** |
m-cresol in chloroform, up to 10% |
Dimethylformamide |
dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene |
o-Dichlorobenzene |
1-chloronaphthalene, trichlorobenzene |
Notes:
1、In case of TSKgel SuperHZ and HXL, keep flow rate below <0.5 mL/min during solvent change. Solvent can be changed one way/one time only.
2、All TSKgel HXL, HHR, SuperHZ and SuperH analytical columns are shipped containing tetrahydrofuran(THF), except High Temperature(HT) columns, which contain o-dichlorobenzene.
3、Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with TSKgel H-type columns; use this solvent with TSKgel SW-type or Alpha-type columns.
** TSKgel H columns may be specially ordered with this shipping solvent.
TSKgel SuperHZ1000
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm |
Pore size(mean): |
1.5 nm |
Exclusion Limit: |
1,000 Da |
Max. Temp.(ºC): |
60 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019309 |
TSKgel SuperHZ1000 |
3 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019309 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019302 |
TSKgel SuperHZ1000 |
3 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019302 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZ2000
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm |
Pore size(mean): |
2.0 nm |
Exclusion Limit: |
10,000 Da |
Max. Temp.(ºC): |
60 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019310 |
TSKgel SuperHZ2000 |
3 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019310 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019303 |
TSKgel SuperHZ2000 |
3 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019303 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZ2500
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm |
Pore size(mean): |
3.0 nm |
Exclusion Limit: |
20,000 Da |
Max. Temp.(ºC): |
60 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019311 |
TSKgel SuperHZ2500 |
3 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019311 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019304 |
TSKgel SuperHZ2500 |
3 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019304 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZ3000
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm |
Pore size(mean): |
7.5 nm |
Exclusion Limit: |
60,000 Da |
Max. Temp.(ºC): |
60 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019312 |
TSKgel SuperHZ3000 |
3 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019312 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019305 |
TSKgel SuperHZ3000 |
3 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019305 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZ4000
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm |
Pore size(mean): |
2.0 nm |
Exclusion Limit: |
4 x 105 Da |
Max. Temp.(ºC): |
80 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019313 |
TSKgel SuperHZ4000 |
3 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019313 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019306 |
TSKgel SuperHZ4000 |
3 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019306 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZM-H
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
10 µm |
Pore size(mean): |
mixed |
Exclusion Limit: |
4 x 108 Da |
Max. Temp.(ºC): |
80 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019664 |
TSKgel SuperHZM-H |
10 µm |
Stainless Steel |
4.6 |
15 |
0019668 |
Guard Column for P/N 0019664 |
10 µm |
Stainless Steel |
4.6 |
2 |
0019665 |
TSKgel SuperHZM-H |
10 µm |
Stainless Steel |
6 |
15 |
0019667 |
Guard Column for P/N 0019665 |
10 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZM-M
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm & 5 µm |
Pore size(mean): |
mixed |
Exclusion Limit: |
4 x 106 Da |
Max. Temp.(ºC): |
80 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019662 |
TSKgel SuperHZM-M |
3 & 5 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019662 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019663 |
TSKgel SuperHZM-M |
3 & 5 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019663 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
TSKgel SuperHZM-N
Matrix: |
polystyrene-divinylbenzene |
Particle Size(mean): |
3 µm |
Pore size(mean): |
mixed |
Exclusion Limit: |
700,000 Da |
Max. Temp.(ºC): |
80 |
Shipping Solvent: |
THF |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019660 |
TSKgelSuperHZM-N |
3 µm |
Stainless Steel |
4.6 |
15 |
0019314 |
Guard Column for P/N 0019660 |
3 µm |
Stainless Steel |
4.6 |
2 |
0019661 |
TSKgelSuperHZM-N |
3 µm |
Stainless Steel |
6 |
15 |
0019666 |
Guard Column for P/N 0019661 |
3 µm |
Stainless Steel |
4.6 |
3.5 |
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TSKgel SuperH Columns
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