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TOSOH Column
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Description
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TSKgel PW Columns for Analysis of Water-Soluble Polymers
TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.
The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.
The figure to the left shows the calibration curves for all TSKgel PW-type columns. Suitable solvents for various polymer samples are shown in the table below.
Specialty TSKgel PW-type Columns
The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples. TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides. TSKgel G1000PW is the preferred column for analysis of low MW oligomers.
Type of Polymer |
Typical Sample |
Suitable Eluent |
||||||
Nonionic hydrophilic |
polyyethylene glycol soluble starch, methyl cellulose, |
distilled water |
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|
polyvinylpyrrolidone |
Buffer or salt solution with organic solvent(e.g. 20% CH3CN in 0.1 mol/L NaNO3) |
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Anionic Hydrophilic |
sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate |
Buffer or salt solution(e.g. 0.1 mol/L NaNO3) |
||||||
Anionic Hydrophobic |
sulfonated lignin sodium salt, |
Buffer or salt solution with organic solvent(e.g. 20% CH3CN in 0.1 mol/L NaNO3) |
||||||
Cationic Hydrophilic |
glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt |
0.5M acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3 |
||||||
Cationic Hydrophobic |
poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt |
0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 |
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Amphoteric Hydrophilic |
peptides, proteins, poly- and oligosaccharides, DNA, RNA |
Buffer or salt solution(e.g. 0.1 mol/L NaNO3) |
||||||
Amphoteric hydrophobic |
blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides |
Buffer or salt solution with organic solvent(e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA) |
TSKgel G2000PW
Base Material: |
hydroxylated polymethacylate |
Particle Size(mean): |
12 µm |
Pore size(mean): |
12.5 nm |
Calibration Range(polyethylene glycols and oxides): |
up to 2,000 Da |
Max. % Organic solvent: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0005761 |
TSKgel G2000PW |
12 µm |
Stainless Steel |
7.5 |
30 |
0005105 |
TSKgel G2000PW |
12 µm |
Stainless Steel |
7.5 |
60 |
0006763 |
Guard Column for P/N 0005761 and 0005105 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
TSKgel G2500PW
Base Material: |
hydroxylated polymethacrylate |
Particle Size(mean): |
12 µm and 17 µm |
Pore size(mean): |
<20 nm |
Calibration Range(polyethylene glycols and oxides): |
up to 3,000 Da |
Max. % Organic: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0008028 |
TSKgel G2500PW |
12 µm |
Stainless Steel |
7.5 |
30 |
0008029 |
TSKgel G2500PW |
12 µm |
Stainless Steel |
7.5 |
60 |
0006762 |
Guard Column for P/N 0008028, 0008029 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
0016248 |
TSKgel G2500PW |
17 µm |
Stainless Steel |
21.5 |
30 |
0008030 |
TSKgel G2500PW |
17 µm |
Stainless Steel |
21.5 |
60 |
0006758 |
Guard Column for P/N 0016248, 0008030 |
17 µm |
Stainless Steel |
21.5 |
7.5 |
TSKgel G3000PW
Base Material: |
hydroxylated polymethacylate |
Particle Size(mean): |
12 µm and 17 µm |
Pore size(mean): |
20 nm |
Calibration Range(polyethylene glycols and oxides): |
up to 50,000 Da |
Max. % Organic: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0005762 |
TSKgel G3000PW |
12 µm |
Stainless Steel |
7.5 |
30 |
0005106 |
TSKgel G3000PW |
12 µm |
Stainless Steel |
7.5 |
60 |
0006762 |
Guard Column for P/N 0005762, 0005106 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
0016249 |
TSKgel G3000PW |
17 µm |
Stainless Steel |
21.5 |
30 |
0005151 |
TSKgel G3000PW |
17 µm |
Stainless Steel |
21.5 |
60 |
0006758 |
Guard Column for P/N 0016249, 0005151 |
17 µm |
Stainless Steel |
21.5 |
7.5 |
TSKgel G4000PW
Base Material: |
hydroxylated polymethacylate |
Particle Size(mean): |
17 µm |
Pore size(mean): |
50 nm |
Calibration Range(polyethylene glycols and oxides): |
up to 300,000 Da |
Max. % Organic: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0005763 |
TSKgel G4000PW |
17 µm |
Stainless Steel |
7.5 |
30 |
0005107 |
TSKgel G4000PW |
17 µm |
Stainless Steel |
7.5 |
60 |
0006762 |
Guard Column for P/N 0005763, 0005107 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
0016250 |
TSKgel G4000PW |
20 µm |
Stainless Steel |
21.5 |
30 |
0005152 |
TSKgel G4000PW |
20 µm |
Stainless Steel |
21.5 |
60 |
0006758 |
Guard Column for P/N 0016250, 0005152 |
17 µm |
Stainless Steel |
21.5 |
7.5 |
TSKgel G5000PW
Base Material: |
hydroxylated polymethacylate |
Particle Size(mean): |
17 µm |
Pore size(mean): |
100 nm |
Calibration Range(polyethylene glycols and oxides): |
up to 1,000,000 Da |
Max. % Organic: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0005764 |
TSKgel G5000PW |
17 µm |
Stainless Steel |
7.5 |
30 |
0005108 |
TSKgel G5000PW |
17 µm |
Stainless Steel |
7.5 |
60 |
0006762 |
Guard Column for P/N 0005764, 0005108 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
0016251 |
TSKgel G5000PW |
20 µm |
Stainless Steel |
21.5 |
30 |
0005153 |
TSKgel G5000PW |
20 µm |
Stainless Steel |
21.5 |
60 |
0006758 |
Guard Column for P/N 0016251, 0005153 |
17 µm |
Stainless Steel |
21.5 |
7.5 |
TSKgel G6000PW
Base Material: |
hydroxylated polymethacylate |
Particle Size(mean): |
17 µm |
Pore size(mean): |
>100 nm |
Calibration Range(polyethylene glycols and oxides): |
up to 8,000,000 Da |
Max. % Organic: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0005765 |
TSKgel G6000PW |
17 µm |
Stainless Steel |
7.5 |
30 |
0005109 |
TSKgel G6000PW |
17 µm |
Stainless Steel |
7.5 |
60 |
0006762 |
Guard Column for P/N 0005765 and 0005109 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
0020024 |
TSKgel BioAssist G6PW |
17 µm |
PEEK |
7.8 |
30 |
TSKgel GMPW
Base Material: |
hydroxylated polymethacylate |
Particle Size(mean): |
17 µm |
Pore size(mean): |
<10 - 100 nm |
Calibration Range(polyethylene glycols and oxides): |
500 - 8,000,000 Da |
Max. % Organic: |
20% |
Shipping Solvent: |
water |
P/N |
Description |
Particlesize |
Housingmaterial |
ID(mm) |
Length(cm) |
0008026 |
TSKgel GMPW |
17 µm |
Stainless Steel |
7.5 |
30 |
0008027 |
TSKgel GMPW |
17 µm |
Stainless Steel |
7.5 |
60 |
0006762 |
Guard Column for P/N 0008026 and 0008027 |
13 µm |
Stainless Steel |
7.5 |
7.5 |
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