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TOSOH Column

TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.
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Description
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TSKgel PW Columns for Analysis of Water-Soluble Polymers

TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.

The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.

The figure to the left shows the calibration curves for all TSKgel PW-type columns. Suitable solvents for various polymer samples are shown in the table below.

Greenherbs

Specialty TSKgel PW-type Columns

The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples. TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides. TSKgel G1000PW is the preferred column for analysis of low MW oligomers.

Type of Polymer

Typical Sample

Suitable Eluent

Nonionic hydrophilic

polyyethylene glycol

soluble starch, methyl cellulose,
pullulan dextran, hydroxyethyl cellulose
polyvinyl alcohol, polyacrylamide

distilled water
0.01 mol/L NaOH
20% DMSO
Buffer or salt solution(e.g. 0.1-0.5 mol/L NaNO3)

Nonionic Hydrophobic

polyvinylpyrrolidone

Buffer or salt solution with organic solvent(e.g. 20% CH3CN in 0.1 mol/L NaNO3)

Anionic Hydrophilic

sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate

Buffer or salt solution(e.g. 0.1 mol/L NaNO3)

Anionic Hydrophobic

sulfonated lignin sodium salt,
sodium polystyrenesulfonate

Buffer or salt solution with organic solvent(e.g. 20% CH3CN in 0.1 mol/L NaNO3)

Cationic Hydrophilic

glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt

0.5M acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3

Cationic Hydrophobic

poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt

0.5 mol/L acetic acid with 0.3 mol/L Na2SO4

Amphoteric Hydrophilic

peptides, proteins, poly- and oligosaccharides, DNA, RNA

Buffer or salt solution(e.g. 0.1 mol/L NaNO3)

Amphoteric hydrophobic

blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides

Buffer or salt solution with organic solvent(e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA)

TSKgel G2000PW

Base Material:

hydroxylated polymethacylate

Particle Size(mean):

12 µm

Pore size(mean):

12.5 nm

Calibration Range(polyethylene glycols and oxides):

up to 2,000 Da

Max. % Organic solvent:

20%

Shipping Solvent:

water

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0005761

TSKgel G2000PW

12 µm

Stainless Steel

7.5

30

0005105

TSKgel G2000PW

12 µm

Stainless Steel

7.5

60

0006763

Guard Column for P/N 0005761 and 0005105

13 µm

Stainless Steel

7.5

7.5

TSKgel G2500PW

Base Material:

hydroxylated polymethacrylate

Particle Size(mean):

12 µm and 17 µm

Pore size(mean):

<20 nm

Calibration Range(polyethylene glycols and oxides):

up to 3,000 Da

Max. % Organic:

20%

Shipping Solvent:

water

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0008028

TSKgel G2500PW

12 µm

Stainless Steel

7.5

30

0008029

TSKgel G2500PW

12 µm

Stainless Steel

7.5

60

0006762

Guard Column for P/N 0008028, 0008029

13 µm

Stainless Steel

7.5

7.5

0016248

TSKgel G2500PW

17 µm

Stainless Steel

21.5

30

0008030

TSKgel G2500PW

17 µm

Stainless Steel

21.5

60

0006758

Guard Column for P/N 0016248, 0008030

17 µm

Stainless Steel

21.5

7.5

TSKgel G3000PW

Base Material:

hydroxylated polymethacylate

Particle Size(mean):

12 µm and 17 µm

Pore size(mean):

20 nm

Calibration Range(polyethylene glycols and oxides):

up to 50,000 Da

Max. % Organic:

20%

Shipping Solvent:

water

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0005762

TSKgel G3000PW

12 µm

Stainless Steel

7.5

30

0005106

TSKgel G3000PW

12 µm

Stainless Steel

7.5

60

0006762

Guard Column for P/N 0005762, 0005106

13 µm

Stainless Steel

7.5

7.5

0016249

TSKgel G3000PW

17 µm

Stainless Steel

21.5

30

0005151

TSKgel G3000PW

17 µm

Stainless Steel

21.5

60

0006758

Guard Column for P/N 0016249, 0005151

17 µm

Stainless Steel

21.5

7.5

TSKgel G4000PW

Base Material:

hydroxylated polymethacylate

Particle Size(mean):

17 µm

Pore size(mean):

50 nm

Calibration Range(polyethylene glycols and oxides):

up to 300,000 Da

Max. % Organic:

20%

Shipping Solvent:

water

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0005763

TSKgel G4000PW

17 µm

Stainless Steel

7.5

30

0005107

TSKgel G4000PW

17 µm

Stainless Steel

7.5

60

0006762

Guard Column for P/N 0005763, 0005107

13 µm

Stainless Steel

7.5

7.5

0016250

TSKgel G4000PW

20 µm

Stainless Steel

21.5

30

0005152

TSKgel G4000PW

20 µm

Stainless Steel

21.5

60

0006758

Guard Column for P/N 0016250, 0005152

17 µm

Stainless Steel

21.5

7.5

TSKgel G5000PW

Base Material:

hydroxylated polymethacylate

Particle Size(mean):

17 µm

Pore size(mean):

100 nm

Calibration Range(polyethylene glycols and oxides):

up to 1,000,000 Da

Max. % Organic:

20%

Shipping Solvent:

water

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0005764

TSKgel G5000PW

17 µm

Stainless Steel

7.5

30

0005108

TSKgel G5000PW

17 µm

Stainless Steel

7.5

60

0006762

Guard Column for P/N 0005764, 0005108

13 µm

Stainless Steel

7.5

7.5

0016251

TSKgel G5000PW

20 µm

Stainless Steel

21.5

30

0005153

TSKgel G5000PW

20 µm

Stainless Steel

21.5

60

0006758

Guard Column for P/N 0016251, 0005153

17 µm

Stainless Steel

21.5

7.5

TSKgel G6000PW

Base Material:

hydroxylated polymethacylate

Particle Size(mean):

17 µm

Pore size(mean):

>100 nm

Calibration Range(polyethylene glycols and oxides):

up to 8,000,000 Da

Max. % Organic:

20%

Shipping Solvent:

water

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0005765

TSKgel G6000PW

17 µm

Stainless Steel

7.5

30

0005109

TSKgel G6000PW

17 µm

Stainless Steel

7.5

60

0006762

Guard Column for P/N 0005765 and 0005109

13 µm

Stainless Steel

7.5

7.5

0020024

TSKgel BioAssist G6PW

17 µm

PEEK

7.8

30

TSKgel GMPW

Base Material:

hydroxylated polymethacylate

Particle Size(mean):

17 µm

Pore size(mean):

<10 - 100 nm

Calibration Range(polyethylene glycols and oxides):

500 - 8,000,000 Da

Max. % Organic:

20%

Shipping Solvent:

water

P/N

Description

Particlesize

Housingmaterial

ID(mm)

Length(cm)

0008026

TSKgel GMPW

17 µm

Stainless Steel

7.5

30

0008027

TSKgel GMPW

17 µm

Stainless Steel

7.5

60

0006762

Guard Column for P/N 0008026 and 0008027

13 µm

Stainless Steel

7.5

7.5

We could not find any corresponding parameters, please add them to the properties table

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