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Size Exclusion Chromatography Resins

Size exclusion chromatography(SEC), also referred to as gel-filtration chromatography, uses porous particles to separate biological molecules of different sizes. Molecules that are smaller than the pore size can enter the particles and therefore have a longer path length and longer residence time than larger molecules that cannot enter the particles.
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Size Exclusion Chromatography Resins

Size exclusion chromatography(SEC), also referred to as gel-filtration chromatography, uses porous particles to separate biological molecules of different sizes. Molecules that are smaller than the pore size can enter the particles and therefore have a longer path length and longer residence time than larger molecules that cannot enter the particles.

Proteins larger than the pore size can not enter the pores and will thus co-elute as the first peak in the chromatogram. These molecules are said to be totally excluded from the pores. Proteins that can at least partially enter the pores will have varying residence times dependent on the formula weight and hydrodynamic radius of each individual protein. Different proteins will therefore have different residence times when passing through the column. Proteins that elute in this portion of a chromatogram are said to be selectively permeable to the pores. Molecules that are smaller than the pore size are able to enter all of the pores in the resin and will therefore have the longest residence times. These molecules typically elute together as the last peak in a chromatogram and are said to be totally permeable to the pores.

The main application of size exclusion chromatography is the fractionation and purification of synthetic and biological polymers, such as proteins, polysaccharides and nucleic acids.

The advantages of this method include good separation of large molecules from the small molecules while preserving the biological activity of the particles to be separated. The technique is generally combined with others that further separate molecules by other characteristics, such as pI, charge, and affinity for certain compounds and is typically used as a polishing step.

Size Exclusion Chromatography

Calibration curves for globular proteins on

TOYOPEARL HW resins

Greenherbs

Typical resolution changes based on

particle size

Greenherbs

TOYOPEARL HW-40

TOYOPEARL HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

TOYOPEARL HW-40 resin is available in three particle sizes.

Pore size(mean)

5 nm

Exclusion limit

10,000 Da

Particle size(mean)

75 µm(C-grade)
45 µm(F-grade)

30 µm(S-grade)

Pressure rating

0.3 MPa

Shipping buffer

20% ethanol

pH stability

2 - 13

P/N

Description

Resin volume

Particle size

0007450

Toyopearl HW-40EC

500 mL

100 - 300 µm

0007449

Toyopearl HW-40C

500 mL

50 - 100 µm

0014683

Toyopearl HW-40C

1 L

50 - 100 µm

0007969

Toyopearl HW-40C

5 L

50 - 100 µm

0007448

Toyopearl HW-40F

500 mL

30 - 60 µm

0014682

Toyopearl HW-40F

1 L

30 - 60 µm

0007968

Toyopearl HW-40F

5 L

30 - 60 µm

0007447

Toyopearl HW-40S

500 mL

20 - 40 µm

0014681

Toyopearl HW-40S

1 L

20 - 40 µm

0007967

Toyopearl HW-40S

5 L

20 - 40 µm

TOYOPEARL HW-50

TOYOPEARL HW-50 resin is a hydroxylated methacrylic polymer for size exclusion chromatography of proteins between 500-80,000 Da.

TOYOPEARL HW-50 resin is available in two particle sizes.

Pore size(mean)

12.5 nm

Exclusion limit

80,000 Da

Particle size(mean)

45 µm(F-grade)
30 µm(S-grade)

Pressure rating

0.3 MPa

Shipping buffer

20% ethanol

pH stability

2 - 13

P/N

Description

Resin volume

Particle size

0007453

Toyopearl HW-50F

500 mL

30 - 60 µm

0014685

Toyopearl HW-50F

1 L

30 - 60 µm

0008060

Toyopearl HW-50F

5 L

30 - 60 µm

0007452

Toyopearl HW-50S

500 mL

20 - 40 µm

0014684

Toyopearl HW-50S

1 L

20 - 40 µm

0008059

Toyopearl HW-50S

5 L

20 - 40 µm

TOYOPEARL HW-55

TOYOPEARL HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all TOYOPEARL "-550" designated products.

TOYOPEARL HW-55 resin is available in two particle sizes.

Pore size(mean)

50 nm

Exclusion limit

700,000 Da

Particle size(mean)

45 µm(F-grade)
30 µm(S-grade)

Pressure rating

0.3 MPa

Shipping buffer

20% ethanol

pH stability

2 - 13

P/N

Description

Resin volume

Particle size

0007457

Toyopearl HW-55F

500 mL

30 - 60 µm

0014687

Toyopearl HW-55F

1 L

30 - 60 µm

0008063

Toyopearl HW-55F

5 L

30 - 60 µm

0007456

Toyopearl HW-55S

500 mL

20 - 40 µm

0014686

Toyopearl HW-55S

1 L

20 - 40 µm

0008062

Toyopearl HW-55S

5 L

20 - 40 µm

TOYOPEARL HW-65

TOYOPEARL HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all TOYOPEARL "-650" designated products.

TOYOPEARL HW-65 SEC resin is available in two particle sizes.

Pore size(mean)

100 nm

Exclusion limit

5,000,000 Da

Particle size(mean)

45 µm(F-grade)
30 µm(S-grade)

Pressure rating

0.3 MPa

Shipping buffer

20% ethanol

pH stability

2 - 13

P/N

Description

Resin volume

Particle size

0007465

Toyopearl HW-65F

500 mL

30 - 60 µm

0014689

Toyopearl HW-65F

1 L

30 - 60 µm

0008069

Toyopearl HW-65F

5 L

30 - 60 µm

0007464

Toyopearl HW-65S

500 mL

20 - 40 µm

0014688

Toyopearl HW-65S

1 L

20 - 40 µm

0008068

Toyopearl HW-65S

5 L

20 - 40 µm

TOYOPEARL HW-75

TOYOPEARL HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.

TOYOPEARL HW-75 resin is available in two particle sizes.

Pore size(mean)

> 100 nm

Exclusion limit

50,000,000 Da

Particle size(mean)

45 µm(F-grade)
30 µm(S-grade)

Pressure rating

0.3 MPa

Shipping buffer

20% ethanol

pH stability

2 - 13

P/N

Description

Resin volume

Particle size

0007469

Toyopearl HW-75F

500 mL

30 - 60 µm

0014691

Toyopearl HW-75F

1 L

30 - 60 µm

0008072

Toyopearl HW-75F

5 L

30 - 60 µm

0007468

Toyopearl HW-75S

500 mL

20 - 40 µm

0008071

Toyopearl HW-75S

5 L

20 - 40 µm

We could not find any corresponding parameters, please add them to the properties table

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