The OA-5000 and OA-6000 series columns perform chiral recognition in a reversed-phase mode by a coordination exchange interaction. OA-5000 chiral ligand is penicillamine coated on ODS silica gel surface. OA-6000 chiral ligand is tartaric acid derivative coated on the ODS silica gel surface, limiting the amount of organic solvent added to the mobile phase, and The mobile phase of the OA-5000 and OA-6000 series columns must contain Cu2 + ions. This series of columns not only effectively separates amino acids, enantiomers of hydroxy acids, but also separates compounds formed by copper chelation, such as aminoalcohols, amines, carboxylic acids, aminolactams and dipeptides. In particular, the OA-5000 column is extremely versatile and the OA-6000 is effective in separating β-amino acids, β-hydroxy acids and hydrophilic alminic acid.