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Cation Exchange Chromatography

Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl(CM) functional group. A counter ion, often Na+ maintains electroneutrality.
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Cation Exchange Chromatography

Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl(CM) functional group. A counter ion, often Na+ maintains electroneutrality.

In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For example, a molecule with a pI of 8.2 is run in a mobile phase buffer at pH 6.0 with the solid support pKa at 1.2 in cation exchange chromatography.

Highlights:

Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large molecular weight proteins.

TSKgel BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK hardware. Other columns are available in glass and stainless steel for analytic, semi-preparative and preparative applications.

Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is approximately double that of the 5PW packings due to the smaller pore size and larger surface area.

TSKgel SP-5PW column available in 2 mm ID format.

Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.

Specialty columns:

Organic acids by ion-exclusion(TSKgel OApak-A and TSKgel SCX)

TSK TSKgel BioAssist S

Base Material:

polymethacrylate

Particle Size(mean):

7 µm and 13 µm

Pore Size(mean):

130 nm

Functional Group:

sulfopropyl

Counter Ion:

Na+

pH Range:

2.0 - 12.0

Protein Capacity :

70 g gamme globulin/L

Small Ion Capacity:

0.1 eq/L

pKa:

2.4

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0019686

TSKgel BioAssist S

7 µm

PEEK

4.6

5

0021411

TSKgel BioAssist S

13 µm

PEEK

10

10

TSKgel CM-2SW

Base Material:

spherical silica

Particle Size(mean):

5 µm

Pore Size(mean):

12.5 nm

Functional Group:

-CH2COO-

Counter Ion:

Na+

pH Range:

2.0 - 7.5

Protein Capacity :

ND

Small Ion Capacity:

> 0.3 meq/g

pKa:

4.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007167

TSKgel CM-2SW

5 µm

Stainless Steel

4.6

25

0007650

Guardgel Kit for P/N 0007167

TSKgel CM-3SW

Base Material:

spherical silica

Particle Size(mean):

10 µm

Pore Size(mean):

25 nm

Functional Group:

-CH2COO-

Counter Ion:

Na+

pH Range:

2.0 - 7.5

Protrein Capacity :

ND

Small Ion Capacity:

> 0.3 meq/g

pKa:

4.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007162

TSKgel CM-3SW

10 µm

Stainless Steel

7.5

7.5

0007650

Guardgel Kit for P/N 0007162

TSKgel CM-5PW

Base Material:

hydroxylated methacrylic polymer

Particle Size(mean):

10 µm and 13 µm

Pore Size(mean):

100 nm

Functional Group:

-CH2COO-

Counter Ion:

Na+

pH Range:

2.0 - 12.0

Protein Capacity :

45 mg Hb/mL

Small Ion Capacity:

> 0.1 eq/L

pKa:

4.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0013068

TSKgel CM-5PW

10 µm

Stainless Steel

7.5

7.5

0013069

Guardgel Kit for P/N 0013068

Stainless Steel

0014021

TSKgel CM-5PW

13 µm

Stainless Steel

21.5

15

0016094

Guardgel Kit for P/N 0014021

Stainless Steel

0014010

TSKgel CM-5PW

10 µm

Glass

5

5

0014011

TSKgel CM-5PW

10 µm

Glass

8

7.5

0014024

Guardgel Kit for P/N 0014011

Glass

0014012

TSKgel CM-5PW

13 µm

Glass

20

15

0014468

Guard Column for P/N 0014012

Glass

20

2

TSKgel CM-STAT

Base Material:

hydrophilic polymer

Particle Size(mean):

7 µm and 10 µm

Pore Size(mean):

non-porous

Functional Group:

carboxymethyl

Counter Ion:

Na+

pH Range:

3.0 - 10.0

Static Binding Capacity
(mg Lysozyme/g dry gel):

ca. 20(7 µm)

ca. 15(10 µm)

Small Ion Capacity:

100 µeq/g dry gel

pKa:

4.9

Hardware:

stainless steel tubing and fittings, PEEK frits

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0021965

TSKgel CM-STAT

10 µm

Stainless Steel

3

3.5

0021966

TSKgel CM-STAT

7 µm

Stainless Steel

4.6

10

TSKgel OApak-A

Base Material:

hydrophilic polymer

Particle Size(mean):

5 µm

Pore Size(mean):

ND

Functional Group:

proprietary

Counter Ion:

H+

pH Range:

2.0 - 7.5

Small Ion Capacity:

> 1.5 eq/L

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0016653

TSKgel OApak-A

5 µm

Stainless Steel

7.8

30

0016654

TSKgel OApak-P Guard column

10 µm

Stainless Steel

6

4

TSKgel SCX

Base Material:

PS-DVB

Particle Size(mean):

5 µm

Pore Size(mean):

6.0 nm

Functional Group:

sulfonic acid

Counter Ion:

H+ and Na+

pH Range:

2 - 12

Small Ion Capacity:

> 1.5 eq/L

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007156

TSKgel SCX(Na+)

5 µm

Stainless Steel

6

15

0007158

TSKgel SCX(H+)

5 µm

Stainless Steel

7.8

30

TSKgel SP-2SW

Particle Size(mean):

5 µm

Pore Size(mean):

12.5 nm

Functional Group:

sulfopropyl

Counter Ion:

Na+

pH Range:

2.0 - 7.5

Protein Capacity

ND

Small Ion Capacity:

> 0.3 eq/L

pKa:

2.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007165

TSKgel SP-2SW

5 µm

Stainless Steel

4.6

25

0007644

Guardgel Kit for P/N 0007165

Stainless Steel

TSKgel SP-5PW

Base Material:

hydroxylated methacrylic polymer

Particle Size(mean):

10, 13, 20 µm

Pore Size(mean):

100 nm

Functional Group:

-(CH2)SO3-

Counter Ion:

Na+

pH Range:

2.0 - 7.5

Protein Capacity :

40 g Hb/L

Small Ion Capacity:

> 0.1 eq/L

pKa:

2.3

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0018758

TSKgel SP-5PW

10 µm

Stainless Steel

2

7.5

0007161

TSKgel SP-5PW

10 µm

Stainless Steel

7.5

7.5

0007211

Guardgel Kit for P/N 0007161

Stainless Steel

0007575

TSKgel SP-5PW

13 µm

Stainless Steel

21.5

15

0016093

Guardgel Kit for P/N 0007575

Stainless Steel

0013062

TSKgel SP-5PW

10 µm

Glass

5

5

0008803

TSKgel SP-5PW

10 µm

Glass

8

7.5

0008807

Guardgel Kit for P/N 08803

Glass

0014017

TSKgel SP-5PW

13 µm

Glass

20

15

0014467

Guardgel Column for P/N 014017

Glass

20

2

TSKgel SP-NPR

Base Material:

hydroxylated methacrylic polymer

Particle Size(mean):

2.5 µm

Pore Size(mean):

non-porous

Functional Group:

-(CH2)SO3-

Counter Ion:

Na+

pH Range:

2.0 - 12.0

Protein Capacity :

5 mg Hb/mL

Small Ion Capacity:

> 0.1 eq/L

pKa:

2.3

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0013076

TSKgel SP-NPR

2.5 µm

Stainless Steel

4.6

3.5

0021942

TSKgel SP-NPR

2.5 µm

Stainless Steel

0.3

5

TSKgel SP-STAT

Base Material:

hydrophilic polymer

Particle Size(mean):

7 µm and 10 µm

Pore Size(mean):

non-porous

Functional Group:

sulfopropyl

Counter Ion:

Na+

pH Range:

3.0 - 10.0

Static Binding Capacity(mg Lysozyme/g dry gel):

ca. 15(7 µm)

ca. 10(10 µm)

Small Ion Capacity:

23 µeg/g dry gel

pKa:

4.0

Hardware:

Stainless Steel tubing & fittings, PEEK frits

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0021963

TSKgel SP-STAT

10 µm

Stainless Steel

3

3.5

0021964

TSKgel SP-STAT

7 µm

Stainless Steel

4.6

10
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