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Cation Exchange Chromatography
Cation Exchange Chromatography
Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl(CM) functional group. A counter ion, often Na+ maintains electroneutrality.
In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For example, a molecule with a pI of 8.2 is run in a mobile phase buffer at pH 6.0 with the solid support pKa at 1.2 in cation exchange chromatography.
Highlights:
Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large molecular weight proteins.
TSKgel BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK hardware. Other columns are available in glass and stainless steel for analytic, semi-preparative and preparative applications.
Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is approximately double that of the 5PW packings due to the smaller pore size and larger surface area.
TSKgel SP-5PW column available in 2 mm ID format.
Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.
Specialty columns:
Organic acids by ion-exclusion(TSKgel OApak-A and TSKgel SCX)
TSK TSKgel BioAssist S
Base Material: |
polymethacrylate |
|
Particle Size(mean): |
7 µm and 13 µm |
|
Pore Size(mean): |
130 nm |
|
Functional Group: |
sulfopropyl |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
70 g gamme globulin/L |
|
Small Ion Capacity: |
0.1 eq/L |
|
pKa: |
2.4 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019686 |
TSKgel BioAssist S |
7 µm |
PEEK |
4.6 |
5 |
0021411 |
TSKgel BioAssist S |
13 µm |
PEEK |
10 |
10 |
TSKgel CM-2SW
Base Material: |
spherical silica |
|
Particle Size(mean): |
5 µm |
|
Pore Size(mean): |
12.5 nm |
|
Functional Group: |
-CH2COO- |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 7.5 |
|
Protein Capacity : |
ND |
|
Small Ion Capacity: |
> 0.3 meq/g |
|
pKa: |
4.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007167 |
TSKgel CM-2SW |
5 µm |
Stainless Steel |
4.6 |
25 |
0007650 |
Guardgel Kit for P/N 0007167 |
TSKgel CM-3SW
Base Material: |
spherical silica |
|
Particle Size(mean): |
10 µm |
|
Pore Size(mean): |
25 nm |
|
Functional Group: |
-CH2COO- |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 7.5 |
|
Protrein Capacity : |
ND |
|
Small Ion Capacity: |
> 0.3 meq/g |
|
pKa: |
4.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007162 |
TSKgel CM-3SW |
10 µm |
Stainless Steel |
7.5 |
7.5 |
0007650 |
Guardgel Kit for P/N 0007162 |
TSKgel CM-5PW
Base Material: |
hydroxylated methacrylic polymer |
|
Particle Size(mean): |
10 µm and 13 µm |
|
Pore Size(mean): |
100 nm |
|
Functional Group: |
-CH2COO- |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
45 mg Hb/mL |
|
Small Ion Capacity: |
> 0.1 eq/L |
|
pKa: |
4.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
|
0013068 |
TSKgel CM-5PW |
10 µm |
Stainless Steel |
7.5 |
7.5 |
|
0013069 |
Guardgel Kit for P/N 0013068 |
Stainless Steel |
||||
0014021 |
TSKgel CM-5PW |
13 µm |
Stainless Steel |
21.5 |
15 |
|
0016094 |
Guardgel Kit for P/N 0014021 |
Stainless Steel |
||||
0014010 |
TSKgel CM-5PW |
10 µm |
Glass |
5 |
5 |
|
0014011 |
TSKgel CM-5PW |
10 µm |
Glass |
8 |
7.5 |
|
0014024 |
Guardgel Kit for P/N 0014011 |
Glass |
||||
0014012 |
TSKgel CM-5PW |
13 µm |
Glass |
20 |
15 |
|
0014468 |
Guard Column for P/N 0014012 |
Glass |
20 |
2 |
TSKgel CM-STAT
Base Material: |
hydrophilic polymer |
|
Particle Size(mean): |
7 µm and 10 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
carboxymethyl |
|
Counter Ion: |
Na+ |
|
pH Range: |
3.0 - 10.0 |
|
Static Binding Capacity |
ca. 20(7 µm) ca. 15(10 µm) |
|
Small Ion Capacity: |
100 µeq/g dry gel |
|
pKa: |
4.9 |
|
Hardware: |
stainless steel tubing and fittings, PEEK frits |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0021965 |
TSKgel CM-STAT |
10 µm |
Stainless Steel |
3 |
3.5 |
0021966 |
TSKgel CM-STAT |
7 µm |
Stainless Steel |
4.6 |
10 |
TSKgel OApak-A
Base Material: |
hydrophilic polymer |
Particle Size(mean): |
5 µm |
Pore Size(mean): |
ND |
Functional Group: |
proprietary |
Counter Ion: |
H+ |
pH Range: |
2.0 - 7.5 |
Small Ion Capacity: |
> 1.5 eq/L |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0016653 |
TSKgel OApak-A |
5 µm |
Stainless Steel |
7.8 |
30 |
0016654 |
TSKgel OApak-P Guard column |
10 µm |
Stainless Steel |
6 |
4 |
TSKgel SCX
Base Material: |
PS-DVB |
Particle Size(mean): |
5 µm |
Pore Size(mean): |
6.0 nm |
Functional Group: |
sulfonic acid |
Counter Ion: |
H+ and Na+ |
pH Range: |
2 - 12 |
Small Ion Capacity: |
> 1.5 eq/L |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007156 |
TSKgel SCX(Na+) |
5 µm |
Stainless Steel |
6 |
15 |
0007158 |
TSKgel SCX(H+) |
5 µm |
Stainless Steel |
7.8 |
30 |
TSKgel SP-2SW
Particle Size(mean): |
5 µm |
|
Pore Size(mean): |
12.5 nm |
|
Functional Group: |
sulfopropyl |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 7.5 |
|
Protein Capacity |
ND |
|
Small Ion Capacity: |
> 0.3 eq/L |
|
pKa: |
2.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007165 |
TSKgel SP-2SW |
5 µm |
Stainless Steel |
4.6 |
25 |
0007644 |
Guardgel Kit for P/N 0007165 |
Stainless Steel |
TSKgel SP-5PW
Base Material: |
hydroxylated methacrylic polymer |
|
Particle Size(mean): |
10, 13, 20 µm |
|
Pore Size(mean): |
100 nm |
|
Functional Group: |
-(CH2)SO3- |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 7.5 |
|
Protein Capacity : |
40 g Hb/L |
|
Small Ion Capacity: |
> 0.1 eq/L |
|
pKa: |
2.3 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0018758 |
TSKgel SP-5PW |
10 µm |
Stainless Steel |
2 |
7.5 |
0007161 |
TSKgel SP-5PW |
10 µm |
Stainless Steel |
7.5 |
7.5 |
0007211 |
Guardgel Kit for P/N 0007161 |
Stainless Steel |
|||
0007575 |
TSKgel SP-5PW |
13 µm |
Stainless Steel |
21.5 |
15 |
0016093 |
Guardgel Kit for P/N 0007575 |
Stainless Steel |
|||
0013062 |
TSKgel SP-5PW |
10 µm |
Glass |
5 |
5 |
0008803 |
TSKgel SP-5PW |
10 µm |
Glass |
8 |
7.5 |
0008807 |
Guardgel Kit for P/N 08803 |
Glass |
|||
0014017 |
TSKgel SP-5PW |
13 µm |
Glass |
20 |
15 |
0014467 |
Guardgel Column for P/N 014017 |
Glass |
20 |
2 |
TSKgel SP-NPR
Base Material: |
hydroxylated methacrylic polymer |
|
Particle Size(mean): |
2.5 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
-(CH2)SO3- |
|
Counter Ion: |
Na+ |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
5 mg Hb/mL |
|
Small Ion Capacity: |
> 0.1 eq/L |
|
pKa: |
2.3 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0013076 |
TSKgel SP-NPR |
2.5 µm |
Stainless Steel |
4.6 |
3.5 |
0021942 |
TSKgel SP-NPR |
2.5 µm |
Stainless Steel |
0.3 |
5 |
TSKgel SP-STAT
Base Material: |
hydrophilic polymer |
|
Particle Size(mean): |
7 µm and 10 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
sulfopropyl |
|
Counter Ion: |
Na+ |
|
pH Range: |
3.0 - 10.0 |
|
Static Binding Capacity(mg Lysozyme/g dry gel): |
ca. 15(7 µm) ca. 10(10 µm) |
|
Small Ion Capacity: |
23 µeg/g dry gel |
|
pKa: |
4.0 |
|
Hardware: |
Stainless Steel tubing & fittings, PEEK frits |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0021963 |
TSKgel SP-STAT |
10 µm |
Stainless Steel |
3 |
3.5 |
0021964 |
TSKgel SP-STAT |
7 µm |
Stainless Steel |
4.6 |
10 |

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