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Anion Exchange Chromatography
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Description
Parameters
Anion Exchange Chromatography
Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE(diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.
In ion exchange chromatography, the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3.
Highlights:
Pore structure and bonding chemistry of TSKgel BioAssist Q columns provides high capacity for small to very large molecular weight proteins and nucleic acids
TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry; effective pore size is smaller for TSKgel SuperQ-5PW
Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the 5PW packings due to the smaller pore size and larger surface area.
TSKgel DEAE-5PW and DEAE-2SW columns are available in 2 mm ID format
Specialty columns for analysis of mono- and disaccharides, sugar alcohols, and organic acids.
TSKgel DNA-NPR columns for PCR fragment analysis
TSKgel BioAssist columns are packed in PEEK hardware, while other columns are available in glass and stainless steel.
Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.
Speciality Columns
Oligonucleotide fragments, PCR reagents(TSKgel DNA-NPR)
Mono- and disaccharides(TSKgel Sugar AXI and TSKgel Sugar AXG)
TSKgel BioAssist Q
Base Material: |
polymethacylate |
|
Particle Size(mean): |
10 µm and 13 µm |
|
Pore Size(mean): |
400 nm |
|
Functional Group: |
polyamine |
|
Counter Ion: |
Cl- |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
70 g BSA/L |
|
Small Ion Capacity: |
0.1 eq/L |
|
pKa: |
9.4 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0019685 |
TSKgel BioAssist Q |
10 µm |
PEEK |
4.6 |
5 |
0021410 |
TSKgel BioAssist Q |
13 µm |
PEEK |
10 |
10 |
TSKgel DEAE-2SW
Base Material: |
hydrophilic silica |
|
Particle Size(mean): |
5 µm |
|
Pore Size(mean): |
12.5 nm |
|
Functional Group: |
CH2CH2N+(C2H5)3 |
|
Counter Ion: |
H2PO4- |
|
pH Range: |
2.0 - 7.5 |
|
Protein Capacity : |
ND |
|
Small Ion Capacity: |
> 0.3 meq/g |
|
pKa: |
11.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0018761 |
TSKgel DEAE-2SW |
5 µm |
Stainless Steel |
2 |
25 |
0007168 |
TSKgel DEAE-2SW |
5 µm |
Stainless Steel |
4.6 |
25 |
TSKgel DEAE-3SW
Base Material: |
hydrophilic silica |
|
Particle Size(mean): |
10 µm |
|
Pore Size(mean): |
25 nm |
|
Functional Group: |
CH2CH2N+(C2H5)3 |
|
Counter Ion: |
Cl- |
|
pH Range: |
2.0 - 7.5 |
|
Protein Capacity |
ND |
|
Small Ion Capacity: |
> 0.3 eq/g |
|
pKa: |
11.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007163 |
TSKgel DEAE-3SW |
10 µm |
Stainless Steel |
7.5 |
7.5 |
0007648 |
Guardgel Kit for P/N 0007163 |
TSKgel DEAE-5PW
Base Material: |
hydroxylated methacrylate polymer |
Particle Size(mean): |
10, 13 and 20 µm |
Pore Size(mean): |
100 nm |
Functional Group: |
CH2CH2N+(C2H5)3 |
Counter Ion: |
Cl- |
pH Range: |
2.0 - 12.0 |
Protein Capacity : |
30 g BSA/L |
Small Ion Capacity: |
0.1 eq/L |
pKa: |
11.5 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0013061 |
TSKgel DEAE-5PW |
10 µm |
Glass |
5 |
5 |
0008802 |
TSKgel DEAE-5PW |
10 µm |
Glass |
8 |
7.5 |
0008806 |
Glass Guardgel Kit for P/N 0013061, 0008802 |
Glass |
|||
0014016 |
TSKgel DEAE-5PW |
13 µm |
Glass |
20 |
15 |
0014466 |
Glass Guard Column for P/N 0014016 |
Glass |
20 |
2 |
|
0018757 |
TSKgel DEAE-5PW |
10 µm |
Stainless Steel |
2 |
7.5 |
0007164 |
TSKgel DEAE-5PW |
10 µm |
Stainless Steel |
7.5 |
7.5 |
0007210 |
Guardgel Kit for P/N 0007164 |
Stainless Steel |
|||
0007574 |
TSKgel DEAE-5PW |
13 µm |
Stainless Steel |
21.5 |
15 |
0016092 |
Guardgel Kit for P/N 0007574 |
Stainless Steel |
TSKgel DEAE-NPR
Base Material: |
hydroxylated methacrylate polymer |
|
Particle Size(mean): |
2.5 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
CH2CH2N+(C2H5)3 |
|
Counter Ion: |
Cl- |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
5 g BSA/L |
|
Small Ion Capacity: |
> 0.1 eq/L |
|
pKa: |
11.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0013075 |
TSKgel DEAE-NPR |
2.5 µm |
Stainless Steel |
4.6 |
3.5 |
0017088 |
Guard Column for P/N 0013075 |
5 µm |
Stainless Steel |
4.6 |
0.5 |
TSKgel DNA-NPR
Base Material: |
hydroxylated methacrylate polymer |
|
Particle Size(mean): |
2.5 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
proprietary |
|
Counter Ion: |
ClO4- |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
5 g BSA/L |
|
Small Ion Capacity: |
0.1 eq/L |
|
pKa: |
11.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0018249 |
TSKgel DNA-NPR |
2.5 µm |
Stainless Steel |
4.6 |
7.5 |
0018253 |
Guard Column for P/N 0018249 |
5 µm |
Stainless Steel |
4.6 |
0.5 |
TSKgel DNA-STAT
Base Material: |
hydrophilic polymer |
|
Particle Size(mean): |
5 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
quarternary ammonium |
|
Counter Ion: |
Cl- |
|
pH Range: |
3.0 - 10.0 |
|
Static Binding Capacity(mg BSA/g dry gel): |
ca. 35(5 µm) |
|
Small Ion Capacity: |
270 µeg/g dry gel |
|
pKa: |
10.5 |
|
Hardware: |
Stainless Steel tubing & fittings, PEEK frits |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0021962 |
TSKgel DNA-STAT |
5 µm |
Stainless Steel |
4.6 |
10 |
TSKgel QAE-2SW
Base Material: |
silica |
|
Particle Size(mean): |
5 µm |
|
Pore Size(mean): |
12.5 nm |
|
Functional Group: |
trimethylamino |
|
Counter Ion: |
H2PO4- |
|
pH Range: |
2.0 - 7.5 |
|
Protein Capacity : |
ND |
|
Small Ion Capacity: |
> 0.3 meq/g |
|
pKa: |
11.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007166 |
TSKgel QAE-2SW |
5 µm |
Stainless Steel |
4.6 |
25 |
0007646 |
Guardgel Kit for P/N 0007166 |
Stainless Steel |
TSKgel Q-STAT
Base Material: |
hydrophilic polymer |
|
Particle Size(mean): |
7 µm and 10 µm |
|
Pore Size(mean): |
non-porous |
|
Functional Group: |
quarternary ammonium |
|
Counter Ion: |
Cl- |
|
pH Range: |
3.0 - 10.0 |
|
Static Binding Capacity |
ca. 25(7 µm) ca. 20(10 µm) |
|
Small Ion Capacity: |
270 µeg/g dry gel |
|
pKa: |
10.5 |
|
Hardware: |
Stainless Steel tubing & fittings, PEEK frits |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0021960 |
TSKgel Q-STAT |
10 µm |
Stainless Steel |
3 |
3.5 |
0021961 |
TSKgel Q-STAT |
7 µm |
Stainless Steel |
4.6 |
10 |
TSKgel SAX
Base Material: |
PS-DVB polymer |
|
Particle Size(mean): |
5 µm |
|
Pore Size(mean): |
6.0 nm |
|
Functional Group: |
trimethylamine |
|
Counter Ion: |
Cl- |
|
pH Range: |
1.0 - 14.0 |
|
Protein Capacity |
ND |
|
Small Ion Capacity: |
1.5 eq/L |
|
pKa: |
12.5 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0007157 |
TSKgel SAX |
5 µm |
Stainless Steel |
6 |
15 |
TSKgel Sugar AXG
Base Material: |
PS-DVB polymer |
|
Particle Size(mean): |
10 µm |
|
Pore Size(mean): |
6.0 nm |
|
Functional Group: |
trimethylamino |
|
Counter Ion: |
HBO32- |
|
pH Range: |
1.0 - 14.0 |
|
Protein Capacity : |
NA |
|
Small Ion Capacity: |
> 1.2 eq/L |
|
pKa: |
12.5 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0008640 |
TSKgel Sugar AXG |
10 µm |
Stainless Steel |
4.6 |
15 |
TSKgel Sugar AXI
Base Material: |
PS-DVB polymer |
|
Particle Size(mean): |
8 µm |
|
Pore Size(mean): |
6.0 nm |
|
Functional Group: |
trimethylamino |
|
Counter Ion: |
HBO32- |
|
pH Range: |
1.0 - 14.0 |
|
Protein Capacity : |
ND |
|
Small Ion Capacity: |
> 1.2 eq/L |
|
pKa: |
12.5 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0008639 |
TSKgel Sugar AXI |
8 µm |
Stainless Steel |
4.6 |
15 |
TSKgel SuperQ-5PW
Base Material: |
polymethacrylate |
|
Particle Size(mean): |
10 µm and 13 µm |
|
Pore Size(mean): |
100 nm |
|
Functional Group: |
trimethylamino |
|
Counter Ion: |
Cl- |
|
pH Range: |
2.0 - 12.0 |
|
Protein Capacity : |
100 g BSA/L |
|
Small Ion Capacity: |
> 0.13 eq/L |
|
pKa: |
12.2 |
P/N |
Description |
Particle size |
Housing material |
ID(mm) |
Length(cm) |
0018386 |
TSKgel SuperQ-5PW |
10 µm |
Glass |
8 |
7.5 |
0018389 |
Guardgel Kit for P/N 0018386 |
Glass |
|||
0018257 |
TSKgel SuperQ-5PW |
10 µm |
Stainless Steel |
7.5 |
7.5 |
0018388 |
Guardgel Kit for P/N 0018257 |
Stainless Steel |
|||
0018387 |
TSKgel SuperQ-5PW |
13 µm |
Stainless Steel |
21.5 |
15 |
0018390 |
Guardgel Kit for P/N 0018387 |
Stainless Steel |
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