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Anion Exchange Chromatography

Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE(diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.
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Anion Exchange Chromatography

Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE(diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.

In ion exchange chromatography, the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3.

Highlights:

Pore structure and bonding chemistry of TSKgel BioAssist Q columns provides high capacity for small to very large molecular weight proteins and nucleic acids

TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry; effective pore size is smaller for TSKgel SuperQ-5PW

Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the 5PW packings due to the smaller pore size and larger surface area.

TSKgel DEAE-5PW and DEAE-2SW columns are available in 2 mm ID format

Specialty columns for analysis of mono- and disaccharides, sugar alcohols, and organic acids.

TSKgel DNA-NPR columns for PCR fragment analysis

TSKgel BioAssist columns are packed in PEEK hardware, while other columns are available in glass and stainless steel.

Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.

Speciality Columns

Oligonucleotide fragments, PCR reagents(TSKgel DNA-NPR)

Mono- and disaccharides(TSKgel Sugar AXI and TSKgel Sugar AXG)

TSKgel BioAssist Q

Base Material:

polymethacylate

Particle Size(mean):

10 µm and 13 µm

Pore Size(mean):

400 nm

Functional Group:

polyamine

Counter Ion:

Cl-

pH Range:

2.0 - 12.0

Protein Capacity :

70 g BSA/L

Small Ion Capacity:

0.1 eq/L

pKa:

9.4

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0019685

TSKgel BioAssist Q

10 µm

PEEK

4.6

5

0021410

TSKgel BioAssist Q

13 µm

PEEK

10

10

TSKgel DEAE-2SW

Base Material:

hydrophilic silica

Particle Size(mean):

5 µm

Pore Size(mean):

12.5 nm

Functional Group:

CH2CH2N+(C2H5)3

Counter Ion:

H2PO4-

pH Range:

2.0 - 7.5

Protein Capacity :

ND

Small Ion Capacity:

> 0.3 meq/g

pKa:

11.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0018761

TSKgel DEAE-2SW

5 µm

Stainless Steel

2

25

0007168

TSKgel DEAE-2SW

5 µm

Stainless Steel

4.6

25

TSKgel DEAE-3SW

Base Material:

hydrophilic silica

Particle Size(mean):

10 µm

Pore Size(mean):

25 nm

Functional Group:

CH2CH2N+(C2H5)3

Counter Ion:

Cl-

pH Range:

2.0 - 7.5

Protein Capacity

ND

Small Ion Capacity:

> 0.3 eq/g

pKa:

11.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007163

TSKgel DEAE-3SW

10 µm

Stainless Steel

7.5

7.5

0007648

Guardgel Kit for P/N 0007163

TSKgel DEAE-5PW

Base Material:

hydroxylated methacrylate polymer

Particle Size(mean):

10, 13 and 20 µm

Pore Size(mean):

100 nm

Functional Group:

CH2CH2N+(C2H5)3

Counter Ion:

Cl-

pH Range:

2.0 - 12.0

Protein Capacity :

30 g BSA/L

Small Ion Capacity:

0.1 eq/L

pKa:

11.5

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0013061

TSKgel DEAE-5PW

10 µm

Glass

5

5

0008802

TSKgel DEAE-5PW

10 µm

Glass

8

7.5

0008806

Glass Guardgel Kit for P/N 0013061, 0008802

Glass

0014016

TSKgel DEAE-5PW

13 µm

Glass

20

15

0014466

Glass Guard Column for P/N 0014016

Glass

20

2

0018757

TSKgel DEAE-5PW

10 µm

Stainless Steel

2

7.5

0007164

TSKgel DEAE-5PW

10 µm

Stainless Steel

7.5

7.5

0007210

Guardgel Kit for P/N 0007164

Stainless Steel

0007574

TSKgel DEAE-5PW

13 µm

Stainless Steel

21.5

15

0016092

Guardgel Kit for P/N 0007574

Stainless Steel

TSKgel DEAE-NPR

Base Material:

hydroxylated methacrylate polymer

Particle Size(mean):

2.5 µm

Pore Size(mean):

non-porous

Functional Group:

CH2CH2N+(C2H5)3

Counter Ion:

Cl-

pH Range:

2.0 - 12.0

Protein Capacity :

5 g BSA/L

Small Ion Capacity:

> 0.1 eq/L

pKa:

11.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0013075

TSKgel DEAE-NPR

2.5 µm

Stainless Steel

4.6

3.5

0017088

Guard Column for P/N 0013075

5 µm

Stainless Steel

4.6

0.5

TSKgel DNA-NPR

Base Material:

hydroxylated methacrylate polymer

Particle Size(mean):

2.5 µm

Pore Size(mean):

non-porous

Functional Group:

proprietary

Counter Ion:

ClO4-

pH Range:

2.0 - 12.0

Protein Capacity :

5 g BSA/L

Small Ion Capacity:

0.1 eq/L

pKa:

11.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0018249

TSKgel DNA-NPR

2.5 µm

Stainless Steel

4.6

7.5

0018253

Guard Column for P/N 0018249

5 µm

Stainless Steel

4.6

0.5

TSKgel DNA-STAT

Base Material:

hydrophilic polymer

Particle Size(mean):

5 µm

Pore Size(mean):

non-porous

Functional Group:

quarternary ammonium

Counter Ion:

Cl-

pH Range:

3.0 - 10.0

Static Binding Capacity(mg BSA/g dry gel):

ca. 35(5 µm)

Small Ion Capacity:

270 µeg/g dry gel

pKa:

10.5

Hardware:

Stainless Steel tubing & fittings, PEEK frits

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0021962

TSKgel DNA-STAT

5 µm

Stainless Steel

4.6

10

TSKgel QAE-2SW

Base Material:

silica

Particle Size(mean):

5 µm

Pore Size(mean):

12.5 nm

Functional Group:

trimethylamino

Counter Ion:

H2PO4-

pH Range:

2.0 - 7.5

Protein Capacity :

ND

Small Ion Capacity:

> 0.3 meq/g

pKa:

11.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007166

TSKgel QAE-2SW

5 µm

Stainless Steel

4.6

25

0007646

Guardgel Kit for P/N 0007166

Stainless Steel

TSKgel Q-STAT

Base Material:

hydrophilic polymer

Particle Size(mean):

7 µm and 10 µm

Pore Size(mean):

non-porous

Functional Group:

quarternary ammonium

Counter Ion:

Cl-

pH Range:

3.0 - 10.0

Static Binding Capacity
(mg BSA/g dry gel):

ca. 25(7 µm)

ca. 20(10 µm)

Small Ion Capacity:

270 µeg/g dry gel

pKa:

10.5

Hardware:

Stainless Steel tubing & fittings, PEEK frits

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0021960

TSKgel Q-STAT

10 µm

Stainless Steel

3

3.5

0021961

TSKgel Q-STAT

7 µm

Stainless Steel

4.6

10

TSKgel SAX

Base Material:

PS-DVB polymer

Particle Size(mean):

5 µm

Pore Size(mean):

6.0 nm

Functional Group:

trimethylamine

Counter Ion:

Cl-

pH Range:

1.0 - 14.0

Protein Capacity

ND

Small Ion Capacity:

1.5 eq/L

pKa:

12.5

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0007157

TSKgel SAX

5 µm

Stainless Steel

6

15

TSKgel Sugar AXG

Base Material:

PS-DVB polymer

Particle Size(mean):

10 µm

Pore Size(mean):

6.0 nm

Functional Group:

trimethylamino

Counter Ion:

HBO32-

pH Range:

1.0 - 14.0

Protein Capacity :

NA

Small Ion Capacity:

> 1.2 eq/L

pKa:

12.5

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0008640

TSKgel Sugar AXG

10 µm

Stainless Steel

4.6

15

TSKgel Sugar AXI

Base Material:

PS-DVB polymer

Particle Size(mean):

8 µm

Pore Size(mean):

6.0 nm

Functional Group:

trimethylamino

Counter Ion:

HBO32-

pH Range:

1.0 - 14.0

Protein Capacity :

ND

Small Ion Capacity:

> 1.2 eq/L

pKa:

12.5

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0008639

TSKgel Sugar AXI

8 µm

Stainless Steel

4.6

15

TSKgel SuperQ-5PW

Base Material:

polymethacrylate

Particle Size(mean):

10 µm and 13 µm

Pore Size(mean):

100 nm

Functional Group:

trimethylamino

Counter Ion:

Cl-

pH Range:

2.0 - 12.0

Protein Capacity :

100 g BSA/L

Small Ion Capacity:

> 0.13 eq/L

pKa:

12.2

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0018386

TSKgel SuperQ-5PW

10 µm

Glass

8

7.5

0018389

Guardgel Kit for P/N 0018386

Glass

0018257

TSKgel SuperQ-5PW

10 µm

Stainless Steel

7.5

7.5

0018388

Guardgel Kit for P/N 0018257

Stainless Steel

0018387

TSKgel SuperQ-5PW

13 µm

Stainless Steel

21.5

15

0018390

Guardgel Kit for P/N 0018387

Stainless Steel

We could not find any corresponding parameters, please add them to the properties table

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