National Hotline: 400-600-6161

1
2
3
4
5
6

Product List

All categories

Online Service

这是描述信息
搜索
Search

Affinity Chromatography

Affinity chromatography(AFC) is performed on a support that is functionalized with a ligand that shows biological affinity for a particular enzyme or other biological molecule.
Retail price
0.0
Market price
0.0
Number of views:
22
Product serial number
Category
AFC
Quantity
-
+
Stock:
0
1
Description
Parameters

Affinity Chromatography

Affinity chromatography(AFC) is performed on a support that is functionalized with a ligand that shows biological affinity for a particular enzyme or other biological molecule.

Almost all biomolecules can be purified on the basis of specific interaction between their chemical or biological structure and a suitable affinity ligand. Typical molecular pairs are antigens and antibodies, enzymes and coenzymes, and sugars with lectins. Thus, affinity chromatography distinguishes itself from, e.g., reversed phase and ion exchange chromatography, in that a highly specific interaction with the protein of interest is the cause for separation or purification.

Although affinity chromatography is not specific, in that no enzyme interacts with only one substrate, it is the most selective method for separating proteins. The selectivity is often based on spatial recognition via a 'lock-and-key' mechanism, as in antibody-antigen interactions.

Less selective general ligands have affinity for a whole class of proteins, which often require other chromatographic methods for purification of individual members. The term bioselective adsorption has been proposed as an alternative name for affinity chromatography.

Affinity Chromatography(AFC) offers the greatest potential specificity and selectivity for the isolation or purification of biomolecules. To enhance this advantage, three group specific ligands and one chemically active functionality are available through Tosoh Bioscience.

All analytical TSKgel AFC columns are based on the well-established 10 micron rigid TSKgel G5000PW resin. This resin features 100 nm pores that have an estimated exclusion limit of < 10 million Da along with excellent stability from pH 2 to 9.

TSKgel Chelate-5PW

Applications: Chelates with Zn+2, Cu+2 and Ni+2 ions to target histidine residues on proteins

Particle Size(mean):

10 µm and 13 µm

Pore Size(mean):

100 nm

Functional Group:

iminodiacetic acid

pH Range:

2.0 - 12.0

Exclusion Limit(base resin, est.):

< 10,000,000 Da globular proteins

Ligand Concentration:

20 mmol/L resin

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0014440

TSKgel Chelate-5PW

10 µm

Glass

5

5

0014441

TSKgel Chelate-5PW

10 µm

Glass

8

7.5

0014442

TSKgel Guardgel kit for P/N 001441

Glass

0008645

TSKgel Chelate-5PW

10 µm

Stainless Steel

7.5

7.5

0008647

TSKgel Guardgel kit for P/N 0008645

Stainless Steel

0008646

TSKgel Chelate-5PW

13 µm

Stainless Steel

21.5

15

0020022

TSKgel BioAssist Chelate

10 µm

PEEK

7.8

5

TSKgel Boronate-5PW

Applications: Cis-diol groups found on carbohydates, glycosolyated proteins, catecholamines.

Particle Size(mean):

10 µm

Pore Size(mean):

100 nm

Functional Group:

m-aminophenyl boronate

pH Range:

2.0 - 9.0

Exclusion Limit(base resin, est.):

< 10,000,000 Da globular proteins

Adsorption Capacity:

40 mmol/L resin(sorbitol)

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0014449

TSKgel Boronate-5PW

10 µm

Glass

5

5

0014450

TSKgel Boronate-5PW

10 µm

Glass

8

7.5

0014451

TSKgel Guardgel kit for P/N 0014450

Glass

0013066

TSKgel Boronate-5PW

10 µm

Stainless Steel

7.5

7.5

0013125

TSKgel Guardgel kit for P/N 0013066

Stainless Steel

TSKgel Heparin-5PW

Applications: Blood clotting factors, glycoproteins, endoglycosidase, hyaluronidase, lipases, growth factors, nucleic acid-binding proteins

Particle Size(mean):

10 µm

Pore Size(mean):

100 nm

Functional Group:

heparin

pH Range:

5.5 - 9.0

Exclusion Limit(base resin, est.):

< 10,000,000 Da globular proteins

Adsorption Capacity for Antithrombin III:

< 1.8 g/L resin

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0014443

TSKgel Heparin-5PW

10 µm

Glass

5

5

0014444

TSKgel Heparin-5PW

10 µm

Glass

8

7.5

001445

TSKgel Guardgel kit for P/N 0014444

Glass

0013064

TSKgel Heparin-5PW

10 µm

Stainless Steel

7.5

7.5

0013121

TSKgel Guardgel kit for P/N 0013064

Stainless Steel

TSKgel Tresyl-5PW

Applications: Reacts with amino or thiol groups, used primarily for attachment of specific antigens for antibody analysis

Particle Size(mean):

10 µm

Pore Size(mean):

100 nm

Active Group:

tresyl

pH Range:

2.0 - 12.0

Exclusion Limit(base resin, est.):

< 10,000,000 Da globular proteins

Ligand Concentration:

ca. 20 µmol/mL resin

Adsorption Capacity:

> 60 mg/g dry resin(coupling capacity / soybean trypsin inhibitor)

P/N

Description

Particle size

Housing material

ID(mm)

Length(cm)

0014455

TSKgel Tresyl-5PW

10 µm

Stainless Steel

6

4

0014456

TSKgel Tresyl-5PW

10 µm

Stainless Steel

7.5

7.5

0014457

TSKgel Tresyl-5PW

10 µm

Glass

5

5

0014458

TSKgel Tresyl-5PW

10 µm

Glass

8

7.5

0016208

TSKgel Tresyl-5PW Guardgel, 2g

10 µm

We could not find any corresponding parameters, please add them to the properties table
Previous
Next

Powered by WWW.300.CN | 京ICP备05010649号 Beijing Greenherbs Science and Technology Development CO.,Ltd.
Add:  Building 4-3, No. 18 Yard, Jingxing Street, Nanshao Town, Changping District, Beijing,China 100081
Tel:
400-600-6161 / 010-51659766 Email: info@greenherbs.com.cn